The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats

TUTIK WRESDIYATI, MADE ASTAWAN, LUSIA YUNI HASTANTI

Abstract


This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group.

Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat

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DOI: http://dx.doi.org/10.4308/hjb.13.3.%25x

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