Antibacterial Activity of Extracellular Protease Isolated From an Algicolous Fungus Xylaria psidii KT30 Against Gram-Positive Bacteria

  • Taufik Indarmawan Department of Aquatic Products Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor
  • Apon Zaenal Mustopa Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong
  • Bugi Ratno Budiarto Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Cibinong
  • Kustiariyah Tarman Department of Aquatic Products Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Bogor Marine Biotechnology Division, Center for Coastal and Marine Resources Studies, Bogor Agricultural University, Bogor
Keywords: bioactive compounds, endophytes, marine fungi, Xylaria psidii, zymogram

Abstract

Infectious diseases became more serious problem for public health in recent years. Although existing antibacterial drugs have been relatively effective, they do not rule out the emergence of resistance to the drug. Therefore, the intensive exploration of new bioactive compounds from natural, especially peptide compounds began in recent decades in order-handling infection. This study aimed to isolate, purify and test the potential application of Xylaria psidii KT30 extracellular protease as antibacterial agent against Gram-positive bacteria. X. psidii KT30, a marine fungus isolated from red seaweed Kappaphycus alvarezii showed antibacterial activity against Bacillus subtilisand Staphylococcus aureus. Antibacterial compounds of this fungus were predicted as a group of proteases. Extracellular protease exhibited an optimum activity when potato dextrose broth was used as cultivation medium. Furthermore, the highest activity of these proteases was found on fungal extract after day 15 of cultivation with value of 2.33 ± 0.19 U/mL. The partial purification of proteases using G-75 column chromatography resulted in 2 groups of fractions and showed protease activity based on zymogram assay. The extracellular proteases obtained from those fractions have 3 patterns of molecular mass based on sodium dodecyl sulfate–polyacrylamide gel electrophoresis which are 56.62, 89.12, 162.18 kDa.

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Published
2016-11-10
Section
Articles