Antimicrobial activity and identification of bioactive compounds of Söfö-söfö (Acmella cf) leaf extract using GC-MS

Maria bintang, Faoziduhu Bawamenewi, Mega Safithri, Fachriyan H Pasaribu

Abstract


Söfö-söfö is a traditional medicinal plant from Nias Island that can cure fever, cough, diarrhea and fungal infections on the skin. However, the scientific basis of these plants is unknown. The aim of this research was to extract the Söfö-söfö leaf by maceration method using two solvents that were 70 % ethanol and ethyl acetate, to test the antimicrobial activity of the extract by Agar diffusion method against Staphylococcus aureus, Eschercia coli, and Candida albican, and analyze secondary metabolite compounds by fitochemistry test and to determine the components of bioactive compounds by GC-MS. The results showed that the best solvent for making Söfö-söfö extract as antimicrobial is ethyl acetate with a minimum inhibitory concentration of 4000 ppm to S. aureus (1.25 ± 0.35 mm), E. coli (1 mm) and C. albican 6000 ppm (1.5 mm). The secondary metabolite compounds of ethyl acetate extract were alkaloids, flavonoids and steroids. Bioactive compounds found in the Söfö-söfö ethyl acetate extract with potential antimicrobial activity were hexadecanoic acid, stigmasterol, neophytadiene, methyl ester, squalene and phytol.


Keywords


antimicrobials; bioactive compounds; phytochemicals; söfö-söfö (acmella cf)

Full Text:

PDF (Indonesian)

References


Amarantini C, Asmara W, Kushadiwijaya H, Sembiring L. 2009. Seleksi bakteri Salmonella typhi dari jaringan darah penderita demam tifoid. Yogyakarta (ID): Prosiding Seminar Nasional Penelitian (hal.13-20).

[AOAC] Association of Official Analytical Chemist. 2006.Official Method of Analysis. Chemist. Washington DC (USA): Assoc. Off.Anal.Chem.

[BPOM RI] Badan Pengawasan Obat dan Makanan Republik Indonesia. 2014. Persyaratan mutu obat tradisional. Jakarta (ID): Perkep BPOM RI.

[Depkes] Departemen Kesehatan. 2005. Pharmaceutical care untuk penyakit infeksi saluran pernapasan. Jakarta (ID): Kemenenterian Kesehatan RI.

Harbone JB. 1987. Metode Fitokimia. Jilid II. Bandung (ID): Penerbit ITB.

Jurnalis YD. 2009. Pola resistensi kuman penyebab diare terhadap antibiotika. Majalah Kedokteran Andalas 1(33):43-46.

Katno. 2008. Penanganan pasca panen tanaman obat. Jakarta (ID): Balai Besar Penelitian dan Pengembangan Tanaman Obat Dan Obat Tradisional.

[Kemenkes] Kementerian Kesehatan. 2011. Situasi diare di Indonesia. Buletin jendela data dan informasi kesehatan. Jakarta (ID): Departemen Kesehatan RI.

Kusmiyati, Agustini NWS. 2007. Antibacterial activity assay from Porphyridium cruentum microalga. Biodiversitas 8(1):48-53.

Mangunwardoyo W, Cahyaningsih E, Usia T. 2009. Ekstraksi dan identifikasi senyawa antimikroba herba meniran (Phyllanthus niruri L.). J. Ilmu Kefarmasian Indonesia 7(2):57-63.

Murtadlo Y, Kusrini D, Fachriyah E. 2013. Isolasi, identifikasi senyawa alkaloid total daun tempuyung (Sonchus arvensis linn) dan uji sitotoksik dengan metode BSLT (Brine Shrimp Lethality Test). Chem. Info. 1(3):79-385.

Naibaho FG. 2015. Aktivitas antimikrob dan identifikasi senyawa bioaktif ekstrak bawang batak (Allium chinense G.Don.) [tesis]. Bogor (ID): Institut Pertanian Bogor.

Made KDN, Made N, Idayani S. 2014. Identifikasi jamur Candida albicans pada usap vagina ibu hamil trimester I, II dan III di Puskesmas II Denpasar Selatan. Klinika Laboratory 2:137-143.

[Permenkes] Peraturan Menteri Kesehatan. 2011. Pedoman umum penggunaan antibiotik. Jakarta (ID): Kementerian Kesehatan RI.

Purwanto S. 2015. Uji aktivitas antibakteri fraksi aktif ekstrak daun senggani (Melastoma malabathricum L) terhadap Escherchia coli. Jurnal Keperawatan Sriwijaya. 2(2):84-92.

Rajeswari G, Murugan M, Mohan VR. 2012. GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae). RJPBCS 3(4):301-308.

Saifudin A. 2014. Senyawa alam metabolit sekunder. Yogyakarta (ID): Penerbit CV Budi Utama

Syafitri NE, Bintang M, Falah S. 2014. Kandungan Fitokimia, total fenol dan total flavonoid ekstrak buah Harendong (Melastoma affine D. Don). Current Biochemistry 1(3):105-115.

Steele D. 2008. The importance of generating evidence on Typhoid fever for implementing vaccination strategies. J. Infect Developing Countries 2(4):250-252.

Sudha T, Chidambarampillai S, Mohan VR. 2013. GC-MS analysis of bioactive components of aerial parts of kirganelia reticulata poir (Euphorbiaceae). J. Curr. Chem. Pharm. Sc. 3(2):113-122.

Tuna MR, Kepel BJ, Leman MA. 2015. Uji daya hambat ekstrak daun sirsak (Annona muricata L.) terhadap pertumbuhan staphylococcus aureus secara in vitro. Pharmacon Jurnal Ilmiah Farmasi 4(4):65-70.

Zainab, Gunanti F, Witasari HA, Edityaningrum CA, Mustofa, Murrukmihadi M. 2016. Penetapan parameter standarisasi non spesifik ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi L.). Jakarta (ID) Prosiding Rakernas dan Pertemuan Ilmiah Tahunan Ikatan Apoteker Indonesia 2016(210-214).




DOI: http://dx.doi.org/10.29244/102%20-%20111

Refbacks

  • There are currently no refbacks.


Editorial Office :

Current Biochemistry
Department of Biochemistry, Bogor Agricultural University
Jln Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Phone/Fax.: +62-251-8423267
E-mail: current.biochemistry@gmail.com