Delignifikasi Batang Kayu Sengon oleh <i>Trametes versicolor</i>

  • Azmi Azhari Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
  • Syamsul Falah Institut Pertanian Bogor
  • Laita Nurjannah Institut Pertanian Bogor
  • Suryani Suryani Institut Pertanian Bogor
  • Maria Bintang Institut Pertanian Bogor

Abstract

Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.

Author Biographies

Azmi Azhari, Department of Biochemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Departemen Biokimia
Syamsul Falah, Institut Pertanian Bogor
Departemen Biokimia
Laita Nurjannah, Institut Pertanian Bogor
Departemen Biokimia
Suryani Suryani, Institut Pertanian Bogor
Departemen Biokimia
Maria Bintang, Institut Pertanian Bogor
Departemen Biokimia

References

Akhtar M, Blanchette RA, Kirk TK. 1997. Fungal delignification and biomechanical pulping of wood. Adv in Biochem Engineering/Biotech 57: 159-195.

Anita SH, Yanto DHY, Fatriasasi W. 2001. Lignin use of isolation from black liquor on the biopulping of Betung bamboo (Dendrocalamus asper) as selective media for white-rot-fungi. Penelitian Hasil Hutan 29: 312-321.

Azhari I. Hartono R. 2005. Pemanfaatan kayu ki Acret (Spatholdea campanulata Beauv) sebagai bahan baku pulp kertas melalui uji turunan dimensi serat. 17(6). Jurnal Komunikasi Penelitian. p-62.

Browning BL. 1967. Methods of Wood Chemistry. Interscience Publ. Vol II. New York. p 393-396.

[BSN] Badan Standardisasi Nasional. 1989. Standar Nasional Indonesia Nomor 1032 tentang Cara Uji Kadar Sari Ekstrak Benzena Dalam Pulp dan Kayu. Jakarta (ID): Indonesia.

[BSN] Badan Standardisasi Nasional. 2008. Standar Nasional Indonesia Nomor 0492 tentang Pulp dan Kayu, Cara Uji Kadar Lignin Metode Klason. Jakarta (ID): Indonesia.

[BSN] Badan Standardisasi Nasional. 2009. Standar Nasional Indonesia Nomor 0444 tentang Pulp, Cara Uji Kadar Selulosa Alfa, Beta dan Gamma. Jakarta (ID): Indonesia.

Camarero S, Bochini P, Galletti GC, Martinez MJ & Martinez AT. 2001. Compositional changes of wheat lignin by a fungal peroxidase analyzed by Pyrolysis-GC-MS. Analytical and Appied Pyrolisis 58-59:413-423.

Cross CF, Bevan EJ. 1912. Researches on Cellulose III. London (GB): Longmans Green.

Daniel G. 2003. Microview of wood under degradation by bacteria and fungi. Wood deterioration and preservation. ACS Symposium series 845, Washington DC, USA

Fadilah, Distantina S, Dwiningsih SR, Marifah DS. 2009. Pengaruh penambahan glukosa dan ekstrak yeast terhadap biodelignifikasi ampas batang aren. Ekulibrium 8 (1) 29-33.

Fatrisari W, Ermawar RA, Falah F, Yanto DHY, Hermiati E.2009. Pulping soda panas terbuka bambu betung dengan praperlakuan fungi pelapuk putih (Pleurotus ostreatus dan Trametes versicolor). Jurnal Ilmu dan Teknologi Hasil Hutan 2(2): 45-50

Fraser RJ. 2005. Intraspesific comparison of Phanerochaete chrysosporium strains: peroxidase production, pollutant degradation and mycelial differentiation [Thesis]. Grahamstown: Rhodes Univ.

Fengel D, Wegener G. 1995. Kayu: Kimia, Ultrastruktur, Reaksi-Reaksi. Gadjah Mada Universty Press: Yogyakarta.

Fitria RA, Ermawar W. Fatriasari T, Fajriutami, Yanto DHY, Falah F, Hermiati E. 2006. Biopulping Bambu Menggunakan Jamur Pelapuk Putih Schizophylum commune. Jurnal Ilmu dan Teknologi Hasil Hutan 2(2):45-50

Fitria. 2008. Pengolahan biomassa berlignoselulosa secara enzimatis dalam pembuatan pulp: studi kepustakaan. Jurnal Teknologi Pertanian 9(2): 69-74.

Foody B, Tolan JS, Bernstein JD. 1999. Pretreatment process for conversion of cellulose to fuel ethanol. U.S pat. No. 6.090.595.

Fraser RJ. 2005. Intraspesific comparison of Phanerochaete chrysosporium strains: peroxidase production, pollutant degradation and mycelial differentiation [Thesis]. Grahamstown: Rhodes Univ

Guerrero DG, Martínez VE, and Almaráz RDLTA. 2011. Cultivation of Trametes versicolor in Mexico. Micologia Aplicada International 23(2) 55-58

Hossain SM, Anantharaman N. 2006. Activity enhancement of ligninolyitic enzymes of Trametes versicolor with bagasse powder. Afri. J. Biotechnol. 5(1): 189-194

Howard RT, Abotsi E, Jansen VREL, Howard S. 2003. Lignosellulose biotechnology: issue of bioconversion and enzyme production, Afr. J. Biotechnol 2, 602-619.

Irawati D, Azwar NR, Syafii W, Artika IM. 2009. Pemanfaan serbuk kayu untuk produksi etanol dengan perlakuan pendahuluan delignifikasi menggunakan jamur Phanerochaete chrysosporium. Jurnal Ilmu Kehutanan 3(1) 13-22

Krisnawati H, Varis E, Kallio M, Kanninen M. 2011. Paraserianthes falcataria (L.) Nielsen: Ecology, Silviculture and Productivity. Bogor: CIFOR. p 23.

Lynd LR, Weimer PJ, van Zyl WH and Pretorius IS. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev. 6(3): 506-577.

Martina A, Yuli N, Sutisna M. 2002. Optimasi beberapa faktor fisik terhadap laju degradasi selulosa kayu albasia Paraserianthes falcataria (L) Nielsen dan karboksimetilselulosa (CMC) serta enzimatik oleh jamur. J Nat Ind 4: 156-

Morris AJ, Byrne TC, Madden JF, Reller AB. 1996. Duration of Incubation of Fungal Cultures. Journal of clinical microbiology 34 (6):1583-1585

Mosai S, Wolfaardt JF, Prior BA, Christov LP. 1999. Evaluation of selected white rot fungi for biosilfite pulping. Bioresource Technology 68: 89-93

Nakasone K. 1990. Mycologia Memoir 15. Michigan: Hafner Pr. p 224-225

Perez J, Munoz D, Rubia TDL, Martinez T. 2002. Biodegradation and biological treatments of cellulose, hemicellulose and lignin: an overview. Int Microbiol. 5:53-63.

Risdianto H, Setiadi T, Suhardi SH, Nipoperbowo W. 2007. Pemilihan spesies jamur dan media amobilisasi untuk produksi enzim lignolitik. Prosiding seminar nasional rekayasa kimia dan proses. ISSN:1411-4216

Shukla OP, Rau UN, Subramanyam SV. 2004. Biopulping and Biobleaching: an energy and environment saving technology for indian pulp and paper industry. India: Newsletter of ISEB vol 10

Siagian RM, Suprapti S, Komarayati S. 2003. Peranan fungi Pelapuk Putih Dalam Proses Biodelignifikasi Kayu Sengon (Paraserianthes falcataria (L) Nielsen). Jurnal Ilmu dan Teknologi Kayu Tropis 1(1) 47-56

Tuomela M, Vikman M, Hatakka A, Itavaara M. 2000. Biodegradation of lignin in a compost environment: a review. Biores Tech 72: 169–183.

Widjaja A, Andriyani S, Pratami AA. 2002. Study of Biodelignification on Sengon and Pine using White Rot-Fungus Phanerochaetae chrysosporium for Development for Pulp and Paper Industries in Indonesia, Proceeding of the Asian Pasific Congress on Chemical Engineering. Christ Church New Zealand

Zeng GM, Yu HY, Huang HL, Huang DL, Chen YN, Huang GH, Li JB. 2006. Laccase activities of soil inhabiting fungus Penicillium simplicissimum in relation to lignin degradation. W J Microbiol and Biotech 22: 317–324

Published
2014-04-19
Section
Articles