The Addition Effects of Glucose as a Co-substrate on Xylitol Production by <i>Candida guilliermondii</i>
AbstractHigh cost production is one of the constraints of the commercial xylitol production due to high energy needed and pure raw materials. Therefore, it is necessary to improve the xylitol production eficiently with lower production cost by using microorganisms. The research objectives were to determine the optimum xylitol production from xylose by metabolism of C. guilliermondii and effect of glucose as a co-substrate in fermentation medium. The ratio of glucose : xylose (g/L) was 1:25, 1:12, 1:5 and 1:2.5 respectively. The xylitol concentration was measured by spectrophotometer method (D-sorbytol/D-xylitol kit). The result showed that the exponential phase of Candida guilliermondii was 12 h to 36 of incubation and optimum of incubation time to produce the highest xylitol was 72 h. The best ratio- of glucose : xylose to produce xylitol was 9 g/L glucose : 45 g/L xylose (1 : 5). The xylitol concentration produced from medium with the addition of glucose was 2.85 g/L. This concentration increased five times compared to that in the medium without addition of glucose that only reached 2.85 g/L. According to this study, the addition of glucose as a co-substrate could increase the xylitol production.
Carvalho W, Canilha L, Silva da SS. 2007. Semi-continuous xylitol production in sugarcane bagasse hydrolysate: effect of nutritional supplementation. Brazilian J Pharma Sci 43.
Gurgel PV, Mancilha IM, Pecanha RP, Siqueira JFM. 199 5. Xylitol recovery from fermented sugarcane bagasse hydrolysate. Bioresource Technology 52: 219-223.
Kiet A, Milgrom P, Rothen M. 2006. Xylitol, sweeteners, and dental caries. Pediatric Dentistry 28: 154-163.
Rao RS, Jyothi CP, Prakasam RS, Sarma PN, Rao LV. 2006. Xylitol production from corn fiber and sugarcane bagasse hydrolysate by Candida tropicalis. Bioresource Technology 97: 1974-1978.
Rosa SMA, Felipe MGA, Silva SS, Vitolo M. 199 8. Xylose reductase production by Candida guilliermondii. Appl Biochem Biotechnol 70-72: 127-135.
Sampaio et al. 2003. Screening of filamentous fungi for production of xylitol from D-xylose. Brazilian Journal of Microbiology 34: 325-328.
Silva DDV, Felipe MGA. 2006. Effect of glucose: xylose ratio on xylose reductase and xylitol dehydrogenase activities from Candida guilliermondii in sugarcane bagasse hydrolysate. J.Chem Technol Biotechnol 81: 1294-1300.
Silva DDV, Mancilha IM, Silva SS, Felipe MGA. 2007. Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate. Brazilian Archive of Biology and Technology 50: 207-215.
Soleimani M, Tabil L, Panigrahi S. 2006. Bioproduction of polyalcohol (xylitol) from lignocellulosic resources: a review. Dept Agri Biores Eng 06-106.
Tochampa, Sirisansaneeyakul S, Vanichsriratana W, Srinophakun P, Bakker H, Chisti Y. 2005. A model of xylitol production by the yeast Candida mogii. Bioprocess Biosyst Eng 28: 175-183.
Tortora GJ, Funke BR, Case CL. 2006. Microbiology: an Introduction 9th ed. San Francisco: Pearson Education.
Uhari M, Kontiokori T, Koskela M, Niemela M. 199 6. Xylitol chewing gum in prevention of acute otitis media: double blind randomized trial. Br Med J 313: 1180-1184.
Yulianto WA. 2001. Pengaruh pH, kadar xilosa dan kadar glukosa terhadap produksi xilitol oleh Candida shehatae WAY 08. Jurnal Teknologi dan Industri Pangan 12: 156-162.