Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia

  • La Ode Sumarlin
  • Anna Muawanah
  • Prita Wardhani
  • . Masitoh
Keywords: anticancer, antioxidant, honey

Abstract

Scientists have reported that honey contains several chemicals which have the function of antibacterial, anti-inflammatory, analgesic, wound healing, anticancer, antioxidant and free radical scavenging activity. In this study focuses on exploring the honey in the local market with anticancer and antioxidant activity. Methods used include BSLT method for potential of anticancer and antioxidant to DPPH (1,1-difenil-2-pikrilhidrazin) method. In addition, using Fourier Transform Infra Red (FTIR) analysis to test the functional groups in the honey. The analysis showed that the samples of honey has potential as an anticancer and antioxidant. Prameters of LC50 values obtained by the method Brine Shrimp Lethaly Test (BSLT) the highest as anticancer found in honey from Bali with a value 1.50 ppm. While the IC50 value obtained from DPPH method showed that honey derived from Papua has the potential antioxidant with IC50 value 5453.75 ppm. The results of the FTIR analysis showed that all of samples have the same in main functional groups. These results also indicate that honey was in the local market (Indonesia) is very potential to be consumed for various purposes, mainly to improve health. 

Downloads

Download data is not yet available.

Author Biographies

La Ode Sumarlin

Program Studi Kimia, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Jalan Ir. H. Juanda No. 95, Ciputat 15412

Anna Muawanah
Program Studi Kimia, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Jalan Ir. H. Juanda No. 95, Ciputat 15412
Prita Wardhani
Program Studi Kimia, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Jalan Ir. H. Juanda No. 95, Ciputat 15412
. Masitoh
Program Studi Kimia, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Jalan Ir. H. Juanda No. 95, Ciputat 15412

References

Abubakar MB, Abdullah WZ, Sulaiman SA, Suen AB. 2012. A review of molecular mechanisms of the anti-leukemic effects of phenolic compounds in honey. International Journal of Molecular Sci. 13(11): 15054-15073.

Ahmed S, Othman NH. 2013. Honey as a Potential Natural Anticancer Agent: A Review of Its Mechanisms. Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine. DOI 10.1155/2013/829070.

Al-Mamary M, Al-Meeri A, Al-Haborib M. 2002. Antioxidant activities and total phenolics of different types of honey. Nutrition Research. 22(9): 1041-1047.

Bogdanov S. 1997. Antibacterial Substances in Honey. Artikel Swiss Bee Research Centre, Switzerland.

Bourgou S, Ksouri R, Bellila A, Skandrani I, Falleh H, Marzouk B. 2008. Phenolic Composition and Biological Activities of Tunisian Nigella sativa L. shoots and roots. Comptes Rendus Biologies. 331(1): 48-55.

Chang H, Mi M, Ling W, Zhu J, Zhang Q, Wei N, Zhou Y, Tang Y, Yuan J. 2008. Structurally related cytotoxic effects of flavonoids on human cancer cells in vitro. Archives of Pharmacal Research. 31(9): 1137-1144.

Duthie GG. 1999. Determination of activity of antioxidants in human subjects. Proceedings of the Nutrition Society. 58(4): 1015-1024.

Fauzi AN, Nur Azmi MN, Yaacob NS. 2011. Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines. Food and Chemical Toxicology. 49(4): 871-878.

Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM. 2009. Antioxidant Activity of Potuguese Honey Samples: Different Contributions of the Entire Honey and Phenolic Extract. Food Chemistry. 114(4): 1438-1443.

Fukuda M, Kobayashi K, Hirono Y, Miyagawa M, Ishida T, Ejiogu EC, Sawai M, Pinkerton KE, Takeuchi M, 2009. Jungle honey enhances immune function and antitumor activity. Evidence-Based Complementary and Alternative Medicine. 1–8.

Fukuda M, Kobayashi K, Hirono Y, Miyagawa M, Ishida T, Ejiogu EC, Sawai M, Pinkerton KE, Takeuchi M. 2011. Jungle honey enhances immune function and antitumor activity. Evidence-Based Complementary and Alternative Medicine. 908743: 8.

Ghasm AA, Othman NH, Khattak MN, Ismail NM, Saini R. 2010. Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines. BMC Complementary and Alternative Medicine. 10: 49.

Gheldof N, Wang XH, Engeseth NJ. 2002. Identification and Quantification of Antioxidant Components of Honeys from Various Floral Sources. Journal of Agricultural and Food Chemistry. 50(21): 5870-5877.

Gheldof N, Engeseth NJ. 2002. Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. Journal of Agricultural and Food Chemistry. 8(50): 3050-3055.

Gheldof N, Wang XH, Engeseth NJ. 2003. Buckwheat honey increases serum antioxidant capacity in humans. Journal of Agricultural and Food Chemistry. 51(5): 1500-1505.

Gribel NV, Pashinski VG. 1990. The antitumor properties of honey. Voprosy Onkologii. 36(6): 704-709.

Hanani E, Mun’im, Abdul, Sekarini R. 2005. Identifikasi Senyawa Antioksidan dalam Spons Callyspongia Sp dari Kepulauan Seribu. Majalah Ilmu Kefarmasian. II(3): 127-133.

Hudaya A. 2010. Uji Antioksidan dan Antibakteri Bunga Kecombrang (Etlingera elatior) terhadap E.coli dan S.aureus sebagai Pangan Fungsional. [Skripsi]. Jakarta (ID): Universitas Islam Negeri Syarif Hidayatullah.

Hutagalung LE. 2008. Perkembangan Perolehan Madu Lebah Hutan (Apis dorsata) oleh Pemanen Madu di Kabupaten Tapanuli Utara. [Skripsi]. Bogor (ID): Institut Pertanian Bogor.

Indiastuti DN, Purwaningsih Sri, Setiawati Y, Cholies N. 2008. Skrinning Pendahuluan Toksisitas Beberapa Tumbuhan Benalu terhadap Larva Udang Artemia salina Leach. Jurnal Ilmu Kefarmasian Indonesia. 6(2): 81-85.

Indrayani L, Soetjipto H, Sihasale L. 2006. Skrining Fitokimia dan Uji Toksisitas Ekstrak Daun Pencut Kuda (Stachytarpheta jamacencis L. Vahl) Terhadap Larva Udang Artemia salina Leach. Journal of Science. 12: 57-61.

Jaganathan SK, Mandal M. 2009. Honey constituents and their apoptotic effect in colon cancer cells. Journal of Api Product and Api Medical Science. 1(2): 29-36.

Juniarti OD, Yuhernita. 2009. Kandungan senyawa kimia, uji toksisitas (BSLT) dan antioksidan (1,1-diphenyl-2-pikrilhydrazyl) dari ekstrak daun saga. Makara Sains. 13(1): 50-54.

Kekuda TRP, Vinayaka KS, Kumar SVP, Sudharshan SJ. 2009. Antioxidant and Antibacterial Activity of Lichen Extracts, Honey and Their Combination. Journal of Pharmacy Research. 2(12): 1875-1878.

Kucuk M, Kolayli S, Karaoǧlu S, Ulusoy E, Baltaci C, Candan F. 2007. Biological Activities and Chemical Composition of Three Honeys of Different Types from Anatolia. Food Chemistry. 100(2): 526-534.

Lusby PE, Coombes AL, Wilkinson JM. 2005. Bactericidal Activity of Different Honeys against Pathogenic Bacteria. Archives of Medical Research. 36(5): 464-467.

Mariod AA, Ibrahim RM, Ismail M, Ismail N. 2009. Antioxidant Activity and Phenolic Content of Phenolic Rich Fractions from Black Cumin (Nigella sativa) seedcake. Food Chemistry. 116(1): 306-312.

Moongkarndi P, Kosem N, Luanratana O, Jongsomboonkusol S, Pongpan N. 2004. Antiproliferative activity of Thai medicinal plant extracts on human breast adenocarcinoma cell line. Fitoterapia. 75(3-4): 375-377.

Noor N, Raja Adil S, Shaukat A, Shahid M. 2014. Antitumor and antioxidant potential of some selected Pakistani honeys. Food Chemistry. 43: 362-366.

Oszmianski J, Lee CY. 1990. Inhibition of polyphenol oxidase activity and browning by honey. Journal of Agricultural and Food Chemistry. 38(10): 1892-1895.

Othman NH. 2012. Honey and Cancer: Sustainable Inverse Relationship Particularly for Developing Nations-A Review Hindawi Publishing Corporation. Evidence-Based Complementary and Alternative Medicine. 2012: 10.

Permana DN, Hj. Lajis, Abas F, Othman AG, Ahmad R, Kitajama M, Takayama H, Aini N. 2003. Antioxidative Constituent of Hedotis Diffusa Wild. Natural Product Sciences. 19: 1-6.

Ramadhani AN. 2009. Uji Toksisitas Akut Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Larva Artemia Salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT). [Laporan Akhir]. Semarang (ID): Universitas Diponegoro.

Rohman A, Riyanto S. 2005. Daya antioksidan ekstrak etanol Daun Kemuning (Murraya paniculata (L) Jack) secara in vitro. Majalah Farmasi Indonesia. 16(3): 136-140.

Samarghandian S, Afshari JT, Davoodi S. 2011. Honey induces apoptosis in renal cell carcinoma. Pharmacognosy Magazine. 7(25): 46-52.

Samarghandian S, Samini F, Taghavi M. 2014. Antiproliferative and cytotoxic properties of honey in human prostate cancer cell line (PC-3): Possible mechanism of cell growth inhibition and apoptosis induction African. Journal of Pharmacy and Pharmacology. 8(1): 9-15.

Sukandar D, Hermanto S, Lestari E. 2007. Uji Toksisitas Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb.) dengan Metode Brine Shrimp Lethality Test (BSLT). [Laporan Penelitian]. Jakarta (ID): Universitas Islam Negeri Syarif Hidayatullah Jakarta.

Tomasin R, Gomes-Marcondes MCC. 2011. Oral administration of Aloe vera and honey reduces walker tumour growth by decreasing cell proliferation and increasing apoptosis in tumour tissue. Phytotherapy Research. 25(4): 619-623.

Tsiapara AV, Jaakkola M, Chinou I, Graikou K, Tolonen T, Virtanen V, Moutsatsou P. 2009. Bioactivity of Greek honey extracts on breast cancer (MCF-7), prostate cancer (PC-3) and endometrial cancer (Ishikawa) cells: profile analysis of extracts. Food Chemistry. 116(3): 702-708.

Turkmen N, Sari F, Poyrazoglu ES, Velioglu YS. 2006. Effects of prolonged heating on antioxidant activity and colour of honey. Food Chemistry. 95(4): 653-657.

Zuhra CF, Juliarti BT, Herlince S. 2008. Aktivitas Antioksidan Senyawa Flavonoid Dari Daun Katuk (Sauropus androgynus (L.) Merr.). Jurnal Biologi Sumatra. 3(1): 7-10.

Published
2015-03-11
How to Cite
SumarlinL. O., MuawanahA., WardhaniP., & Masitoh. (2015). Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia. Jurnal Ilmu Pertanian Indonesia, 19(3), 136-144. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/9147