Pencirian Mananase Streptomyces costaricanus 451-3

Abstract

Major component of hemicelluloses are mannans (softwoods) and xylans (hardwoods). Hemicclluloses arc used by microbes as a carbon sources. Mannanase and xylanasc arc enzyme complex that are able to degrade hemicelluloses. Mannanase activity from Streptomyces costarianus 451-3 was tested in locust bean gum 0.5% and coconut meal 0.5% medium and was detected by dinitrosalysilic acid method. Protein concentration was measured using Bradford method. Mannanase and xylanase activity were also detected using birchwood xylan and oatspelt xylan medium. The optimum temperature and pH of Streptomyces mannanase strain 451-3 was 40 ^oC and 6,0, respectively. The addition 1mM of Mg²⁺ and Zn²⁺ at final concentration increased the mannanase activity for about 30% and 80%, while 1mM Mn²⁺, Ca²⁺ and Co²⁺ decreased its activity for about 67%, 100%, and 60%, respectively. The addition of I mM ethylene diamine tetraacetic acid tend to decreased the enzyme activity to 30%. The medium which contain birchwood xylan dan oatspelt xylan could induce mannanase activity, hut in a lower degree then that of xylanase.

Keywords: mananase, streptomyces, xilanasc