Uji Aktivitas Lakase dan Selulase pada Lignoselulosa Gambut dengan Berbagai Kadar Air

Ronny Mulyawan, Lilik Tri Indriyati, Happy Widiastuti, Supiandi Sabiham


The degradation of lignocellulose as the main constituents of peat is catalyzed by enzymes such as laccase or cellulase. The purpose of this research was to study the pattern of laccase and cellulase activities on sterile and non-sterile peat materials at three different water contents (125-175, 225-275, and 325-375%). The peat soil used was collected from oil palm rhizosphere in Riau Province. This research used the descriptive method by comparing the mean values between treatments. The results showed that enzymes activities on sterile and non-sterile peat added with laccase or cellulase were higher compared to those without enzyme addition. The highest laccase activity was at the first day of incubation, while that for selulase was at the 10th days of incubation. The activity of enzyme then decreased with the increase in the respected incubation time. The difference in decreasing of lignin and cellulose content at 125-175% water content was higher than at the other water contents. The decreases in lignin and cellulose contents were higher by addition of respected enzymes both in sterile and non-sterile peat. It could be concluded that at higher water content, laccase and cellulase activities were depressed both on sterile and non sterile peat, especially after the addition of enzyme.


cellulose, degradation, lignin, rhizosphere

Full Text:



Ayeni AO, Adeeyo OA, Oresegun OM, Oladimeji TE. 2015. Compositional analysis of lignocellulosic materials: Evaluation of an economically viable method suitable for woody and non-woody biomass. American Journal of Engineering Research. 4(4): 14-19.

Balan V, Bals B, Chundawat SPS, Marshall D, Dale BE. 2009. Lignocellulose biomass treatment using AFEX. Method in Molecular Biology. 581: 61-77. https://doi.org/10.1007/978-1-60761-214-8_5

Blonka E. 2010. Enzyme activity in forest peat soil. Folia Forestali Poloniza. 52(1): 20-25.

Brzezinâska M, Stepniewska Z, Stepniewski M. 1998. Soil oxygen status and dehydrogenase activity. Soil Biology Biochemestry. 30(13): 1783-1790. https://doi.org/10.1016/S0038-0717(98)00043-1

Chasanah E, Dini IR, Mubarik NR. 2013. Karakterisasi enzim selulase Pmp 0126y dari limbah pengolahan agar. Jurnal Pascapanen dan Bioteknologi Perikanan. 8(2): 103-114. https://doi.org/10.15578/ jpbkp.v8i2.41

Dong WY, Zhang XY, Liu XY, Fu XL, Chen FS, Wang HM, Sun XM, Wen XF. 2015. Responses of soil microbial communities and enzyme activities to nitrogen and phosphorus additions in Chinese fir plantations of subtropical China. Biogeosciences. 12: 5537-5546. https://doi.org/10.5194/bg-12-5537-2015

Eichlerova IJ, Snajdr P, Baldrian. 2012. Laccase activity in soils. Considerations for the measurement of enzyme activity. Elsivier Ltd. Chemosphere. 88(10): 1154-1160. https://doi.org/ 10.1016/j.chemosphere.2012.03.019

German DP, Weintraub MN, Grandy AS, Lauber CL, Rinkes ZL, Allison SD. 2011. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biology Biochemestry. 43. 1387-1397. https://doi.org/10.1016/j.soilbio.2011. 03.017

Ghose TK. 1987. Measurement of cellulase activities. Pure and Applied Chemestry. 59: 257-268. https://doi.org/10.1351/pac198759020257

Gianfreda L, Bollag JM. 1996. Influence of natural and anthropogenic factor on enzyme activity in soil. In G. Stotzky and J. M. Bollag (eds). Soil Biochemistry. Marcel Dekker. 9: 123-176.

Harianti M. 2017. Karakter enzim di rhizosfer kelapa sawit pada lahan gambut. [Disertasi]. Bogor (ID): Institut Pertanian Bogor.

Hendriks ATWM, Zeeman G. 2009. Pretreatments to enhance the digestibility of lignocellulose biomass. Bioresource Technology. 100: 10-18. https:// doi.org/10.1016/j.biortech.2008.05.027

Hofrichter M. 2002. Lignin Conversion by Manganese Peroxidase (MnP). Enzyme Microbiology and Technology. 30: 454466. https://doi.org/ 10.1016/S0141-0229(01)00528-2

Kunamneni A, Ballesteros A, Plou FJ, Alcalde M. 2007. Fungal laccase: a versatile enzyme for biotechnological applications. Communicating Current Research and Educational Topics and Trends in Applied Microbiology. A. Méndez-Vilas (Ed.) Formatex. 233-245.

Lyla PS, Ajmal KS. 2006. Marine microbial diversity and ecology: importance and future perspectives. Current Science. 90: 1325-1335.

Malherbe S, Cloete TE. 2002. Lignocellulose biodegradation: fundamentals and applications. Reviews Environmental Sciences Biotecnology. 1: 105-114. https://doi.org/10.1023/A:1020858910 646

Moore E, Landecker. 1996. Fundamentals of the fungi. New Jersey (US): Prenite Hall Inc.

Putri TTA, Lailan S, Anshari GZ. 2016. Emisi karbon dioksida (CO2) rhizosfer dan non rhizosfer dari perkebunan kelapa sawit (Elaeis guineensis) pada lahan gambut dangkal. Jurnal Tanah dan Iklim. 40(1): 43-50.

Roni KM. 2015. Pembuatan bioetanol dari tanah gambut dengan proses hidrolisis asam kuat. Jurnal Berkala Teknik. 5(1): 801-813.

Saropah DA, Jannah A, Maunatin A. 2012. Kinetika reaksi enzimatis ekstrak kasar enzim selulase bakteri selulolitik hasil isolasi dari bekatul. Alchemy. 2(1): 34-35.

Stoilova I, Krastanov A, Stanchev V. 2010. Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation. Advances in Biosciences and Biotechnology. 1: 208-215. https://doi.org/10.4236/abb.2010.13029

Wahyuni NKL, Wirajana IN, Suyasa IWB. 2014. Pengaruh toluena dan waktu inkubasi terhadap aktivitas selulase dari tanah hutan mangrove. Cakra kimia: Indonesia E-Journal of Applied Chemistry. 2(2): 14-19.

Wiedermann MM, Kane ES, Potvin LR, Lilleskov EA. 2017. Interactive plant functional group and water table effects on decomposition and extracellular enzyme activity in Sphagnum peatlands. Soil Biology & Biochemistry. 108: 1-8. https://doi.org/ 10.1016/j.soilbio.2017.01.008

Winarsih S. 2013. Pemanfaatan Jerami Padi untuk Produksi Bioetanol dengan Pretreatment Microwave Alkali dan Hidrolisis Menggunakan Enzim Kasar dari Trichoderma reesei dan Aspergillus niger. Malang (ID): Universitas Brawijaya.

Yuan X, Tian G, Zhao Y, Zhao L, Wang H, Ng TB. 2015. Biochemical characteristics of three laccase isoforms from the Basidiomycete Pleurotus nebrodensis. Journal Molecules. 21: 2-15.

Yulipriyanto H. 2010. Biologi Tanah dan Strategi Pengelolaanya. Yogyakarta (ID). Graha Ilmu.

DOI: http://dx.doi.org/10.18343/jipi.24.1.20

View JIPI Stats


   Creative Commons License

This journal is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License.

Editorial Office: Institute for Research and Community Services (LPPM), Andi Hakim Nasoetion Building, 5th Floor, Jl. Raya Darmaga, IPB Darmaga Campus, Bogor, West Java, Indonesia 16680, Telp/Fax: +62251-8622323, email: jipi-lppm@apps.ipb.ac.id; jipi-lppm@ipb.ac.id